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anti plzf  (R&D Systems)


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    Structured Review

    R&D Systems anti plzf
    Anti Plzf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+plzf/Human+PLZF+Antibody/pmc13079447-66-28-31
    Average 93 stars, based on 89 article reviews
    anti plzf - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Age-Dependent Clonal Expansion of Non-Sperm-Forming Spermatogonial Stem Cells in Mouse Testes.
    Article Snippet: .. They were then cryo- embedded in OCT compound (4583, Sakura Finetek Japan, Tokyo, Japan), cut into 10- μm sections using a CM1520 cryostat (Leica, Wetzlar, Germany), dried for 30 min, and washed in PBS at 4°C for 20 min. After blocking, the sections were incubated with anti- GFRα1 (1∶1000 dilution), anti- GFP (1:300 dilution; A11122, Invitrogen, Waltham, MA, USA), anti- GFP (1:1000 dilution; ab13970, Abcam, Cambridge, UK), anti- Ki67 (1:1000 dilution; ab15580, Abcam), anti- COPS5 (1:50 dilution; 6895, Cell Signaling Technology), anti- EGR4 (1:50 dilution; ab198197, Abcam), and anti- PLZF (1:1000 dilution; AF2944, R&D Systems, Minneapolis, MN, USA) antibodies at 4°C for 12 h. The reaction was visualized with donkey antirabbit IgG Alexa Fluor 488 (1:400 dilution; A21206, Invitrogen), donkey anti- goat IgG Alexa Fluor 594 (1:400 dilution; A11058, Invitrogen), donkey anti- chicken IgG Alexa Fluor 488 (1:400 dilution; A78948, Invitrogen), donkey anti- rabbit IgG Alexa Fluor 594 (1:400 dilution; A21207, Invitrogen), donkey anti- goat IgG Alexa Fluor Plus 405 (1:400 dilution; A48259, Invitrogen), and Hoechst 33342 (1:5000; 19,172–51, Nacalai Tesque, Kyoto, Japan). .. Observations were made, and photographs were taken using a BX51 upright fluorescence microscope equipped with a DP74 CCD camera or an FV3000 confocal laser scanning microscope (Olympus).

    Article Title: Age‐Dependent Clonal Expansion of Non–Sperm‐Forming Spermatogonial Stem Cells in Mouse Testes
    Article Snippet: .. They were then cryo‐embedded in OCT compound (4583, Sakura Finetek Japan, Tokyo, Japan), cut into 10‐μm sections using a CM1520 cryostat (Leica, Wetzlar, Germany), dried for 30 min, and washed in PBS at 4°C for 20 min. After blocking, the sections were incubated with anti‐GFRα1 (1∶1000 dilution), anti‐GFP (1:300 dilution; A11122, Invitrogen, Waltham, MA, USA), anti‐GFP (1:1000 dilution; ab13970, Abcam, Cambridge, UK), anti‐Ki67 (1:1000 dilution; ab15580, Abcam), anti‐COPS5 (1:50 dilution; 6895, Cell Signaling Technology), anti‐EGR4 (1:50 dilution; ab198197, Abcam), and anti‐PLZF (1:1000 dilution; AF2944, R&D Systems, Minneapolis, MN, USA) antibodies at 4°C for 12 h. The reaction was visualized with donkey anti‐rabbit IgG Alexa Fluor 488 (1:400 dilution; A21206, Invitrogen), donkey anti‐goat IgG Alexa Fluor 594 (1:400 dilution; A11058, Invitrogen), donkey anti‐chicken IgG Alexa Fluor 488 (1:400 dilution; A78948, Invitrogen), donkey anti‐rabbit IgG Alexa Fluor 594 (1:400 dilution; A21207, Invitrogen), donkey anti‐goat IgG Alexa Fluor Plus 405 (1:400 dilution; A48259 , Invitrogen), and Hoechst 33342 (1:5000; 19,172–51, Nacalai Tesque, Kyoto, Japan). .. Observations were made, and photographs were taken using a BX51 upright fluorescence microscope equipped with a DP74 CCD camera or an FV3000 confocal laser scanning microscope (Olympus).

    Incubation:

    Article Title: Age-Dependent Clonal Expansion of Non-Sperm-Forming Spermatogonial Stem Cells in Mouse Testes.
    Article Snippet: .. They were then cryo- embedded in OCT compound (4583, Sakura Finetek Japan, Tokyo, Japan), cut into 10- μm sections using a CM1520 cryostat (Leica, Wetzlar, Germany), dried for 30 min, and washed in PBS at 4°C for 20 min. After blocking, the sections were incubated with anti- GFRα1 (1∶1000 dilution), anti- GFP (1:300 dilution; A11122, Invitrogen, Waltham, MA, USA), anti- GFP (1:1000 dilution; ab13970, Abcam, Cambridge, UK), anti- Ki67 (1:1000 dilution; ab15580, Abcam), anti- COPS5 (1:50 dilution; 6895, Cell Signaling Technology), anti- EGR4 (1:50 dilution; ab198197, Abcam), and anti- PLZF (1:1000 dilution; AF2944, R&D Systems, Minneapolis, MN, USA) antibodies at 4°C for 12 h. The reaction was visualized with donkey antirabbit IgG Alexa Fluor 488 (1:400 dilution; A21206, Invitrogen), donkey anti- goat IgG Alexa Fluor 594 (1:400 dilution; A11058, Invitrogen), donkey anti- chicken IgG Alexa Fluor 488 (1:400 dilution; A78948, Invitrogen), donkey anti- rabbit IgG Alexa Fluor 594 (1:400 dilution; A21207, Invitrogen), donkey anti- goat IgG Alexa Fluor Plus 405 (1:400 dilution; A48259, Invitrogen), and Hoechst 33342 (1:5000; 19,172–51, Nacalai Tesque, Kyoto, Japan). .. Observations were made, and photographs were taken using a BX51 upright fluorescence microscope equipped with a DP74 CCD camera or an FV3000 confocal laser scanning microscope (Olympus).

    Article Title: Undifferentiated spermatogonia modulate their behavior via the expression of basement membrane protein laminin
    Article Snippet: Sections were blocked for 1 h at room temperature using a solution of 4% donkey serum (Sigma–Aldrich, St. Louis, MO, USA: D9663) in Blocking One (Nacalai Tesque). .. Primary antibodies were incubated overnight at 4°C in 1:1 PBS/Blocking One with anti-GFRα1 (1∶1000 dilution; R&D Systems, Minneapolis, MN, USA: AF560), anti-Lam1 + 2 (1:500 dilution; Abcam: ab7463), anti-PLZF (1:1000 dilution; R&D Systems: AF2944), anti-Rarg (1:500 dilution; Cell Signaling Technology, Danvers, MA, USA: #8965), anti-PH3 (1:500 dilution; Merck, Darmstadt, Germany: 06-570), anti-cKit (1:1000 dilution; R&D Systems: AF1356), anti-Laminin gamma 1 clone A5 (1:50 dilution; Sigma Aldrich, Burlington, MA, USA: MAB1914P), and anti-GFP (1:1000 dilution; Abcam: ab13970). .. After three 10-min washing in PBST (0.1% Tween-20 [Nacalai Tesque] in PBS), the sections were incubated with secondary antibodies for 1 h at room temperature.

    Article Title: Age‐Dependent Clonal Expansion of Non–Sperm‐Forming Spermatogonial Stem Cells in Mouse Testes
    Article Snippet: .. They were then cryo‐embedded in OCT compound (4583, Sakura Finetek Japan, Tokyo, Japan), cut into 10‐μm sections using a CM1520 cryostat (Leica, Wetzlar, Germany), dried for 30 min, and washed in PBS at 4°C for 20 min. After blocking, the sections were incubated with anti‐GFRα1 (1∶1000 dilution), anti‐GFP (1:300 dilution; A11122, Invitrogen, Waltham, MA, USA), anti‐GFP (1:1000 dilution; ab13970, Abcam, Cambridge, UK), anti‐Ki67 (1:1000 dilution; ab15580, Abcam), anti‐COPS5 (1:50 dilution; 6895, Cell Signaling Technology), anti‐EGR4 (1:50 dilution; ab198197, Abcam), and anti‐PLZF (1:1000 dilution; AF2944, R&D Systems, Minneapolis, MN, USA) antibodies at 4°C for 12 h. The reaction was visualized with donkey anti‐rabbit IgG Alexa Fluor 488 (1:400 dilution; A21206, Invitrogen), donkey anti‐goat IgG Alexa Fluor 594 (1:400 dilution; A11058, Invitrogen), donkey anti‐chicken IgG Alexa Fluor 488 (1:400 dilution; A78948, Invitrogen), donkey anti‐rabbit IgG Alexa Fluor 594 (1:400 dilution; A21207, Invitrogen), donkey anti‐goat IgG Alexa Fluor Plus 405 (1:400 dilution; A48259 , Invitrogen), and Hoechst 33342 (1:5000; 19,172–51, Nacalai Tesque, Kyoto, Japan). .. Observations were made, and photographs were taken using a BX51 upright fluorescence microscope equipped with a DP74 CCD camera or an FV3000 confocal laser scanning microscope (Olympus).

    Article Title: DNAJB7 is dispensable for male fertility in mice.
    Article Snippet: .. Sections were blocked in 10% goat serum and incubated with the following primary antibodies: anti-PLZF (diluted 1:100 in TBST, AF-2944, R&D Systems, USA) anti-γH2AX (diluted 1:100 in TBST, 16-202A, Merck Millipore, USA) and anti-SOX9 (diluted 1:100 in TBST, AB5535, Merck Millipore, USA). .. Nuclear DNA and acrosomes were stained with 4’,6-diamidino-2-phenylindole (DAPI, F6057, Sigma– Aldrich, USA) and FITC-conjugated peanut agglutinin (PNA, RL-1072, Vector Labs, USA), respectively.

    Article Title: Undifferentiated Spermatogonia Modulate Their Behavior via the Expression of Basement Membrane Protein Laminin†.
    Article Snippet: Sections were blocked for 1 h at 188 room temperature using a solution of 4% donkey serum (Sigma-Aldrich, St. Louis, MO, USA: D9663) in 189 Blocking One (Nacalai Tesque). .. Primary antibodies were incubated overnight at 4°C in 1:1 PBS/Blocking 190 One with anti-GFRα1 (1∶1000 dilution; R&D Systems, Minneapolis, MN, USA: AF560), anti-Lam1+2 191 (1:500 dilution; Abcam: ab7463), anti-PLZF (1:1000 dilution; R&D Systems: AF2944), anti-Rarg (1:500 192 dilution; Cell Signaling Technology, Danvers, MA, USA: #8965), anti-PH3 (1:500 dilution; Merck, 193 Darmstadt, Germany: 06-570), anti-cKit (1:1000 dilution; R&D Systems: AF1356), anti-Laminin gamma 194 1 clone A5 (1:50 dilution; Sigma Aldrich, Burlington, MA, USA: MAB1914P), and anti-GFP (1:1000 195 dilution; Abcam: ab13970). .. After three 10-min washing in PBST (0.1% Tween-20 [Nacalai Tesque] in PBS), 196 the sections were incubated with secondary antibodies for 1 h at room temperature.

    Staining:

    Article Title: A behind-the-scenes role of BDNF in the survival and differentiation of spermatogonia.
    Article Snippet: .. TSA Biotin System (Perkin Elmer, Boston, MA, USA) was used to amplify signals for the c-Kit proto-oncogene (KIT) staining, as reported previously.19 The following antibodies were used with indicated dilutions: anti-PLZF (1:200; AF2944; R&D systems, Minneapolis, MN, USA), anti-KIT (1:1500; ab112177; Abcam, Cambridge, MA, USA), Alexa 488 goat IgG (1:200; A11034; Life Technologies, Grand Island, NY, USA), and Biotin rat IgG (1:1000; 712-066-153; Jackson ImmunoResearch, West Grove, PA, USA). .. DNA was stained with 4',6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich).



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    Image Search Results


    Auto-exo reduce bone loss and promote osteoblast differentiation in vivo . (A) Schematic diagram of animal experimental design. (B) Quantitative analysis of bone mineral density (BMD) among different groups. (C) HE staining of bone tissues. Scale bar = 100 μm. (D) TRAP staining. Scale bar = 100 μm. (E, F) The expression of osteoblast differentiation-associated genes (RUNX2, OSX, Plzf, Dlx5, and ALP) in OVX mice after Auto-exo treatment were assayed by qRT-PCR (E) and western blot (F). Results are displayed as mean ± SD. ∗ p < 0.05. ∗∗ p < 0.01.

    Journal: Biomedical Journal

    Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism

    doi: 10.1016/j.bj.2025.100870

    Figure Lengend Snippet: Auto-exo reduce bone loss and promote osteoblast differentiation in vivo . (A) Schematic diagram of animal experimental design. (B) Quantitative analysis of bone mineral density (BMD) among different groups. (C) HE staining of bone tissues. Scale bar = 100 μm. (D) TRAP staining. Scale bar = 100 μm. (E, F) The expression of osteoblast differentiation-associated genes (RUNX2, OSX, Plzf, Dlx5, and ALP) in OVX mice after Auto-exo treatment were assayed by qRT-PCR (E) and western blot (F). Results are displayed as mean ± SD. ∗ p < 0.05. ∗∗ p < 0.01.

    Article Snippet: Following blocking with TBST solution with 5 % skim milk, primary antibodies against CD63 (1:1000, ab216130, Abcam), TSG101 (1:5000, ab125011, Abcam), Calnexin (1:20000, ab92573, Abcam), RUNX2 (1:1000, 20700-1-AP, Proteintech), OSX (1:1000, 28694-1-AP, Proteintech), Plzf (1:5000, 66672-1-Ig, Proteintech), Dlx5 (1:1000, 10592-1-AP, Proteintech), ALP, (1:200, sc-271431, Santa cruz) and β-actin (1:2000, 60008-1-lg, Proteintech) were appended to incubate membranes overnight at 4 °C.

    Techniques: In Vivo, Staining, Expressing, Quantitative RT-PCR, Western Blot

    Bilirubin inhibits osteoblast differentiation of MC3T3-E1 cells. (A) ALP staining. (B) The calcified cells were examined by Alizarin red S staining. (C, D) The expression of osteoblast differentiation-associated genes (RUNX2, OSX, Plzf, Dlx5, and ALP) was assayed by qRT-PCR (C) and western blot (D). NC represents normal control. BL represents low concentration of bilirubin. BM represents medium concentration of bilirubin. BH represents high concentration of bilirubin. Results are displayed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01.

    Journal: Biomedical Journal

    Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism

    doi: 10.1016/j.bj.2025.100870

    Figure Lengend Snippet: Bilirubin inhibits osteoblast differentiation of MC3T3-E1 cells. (A) ALP staining. (B) The calcified cells were examined by Alizarin red S staining. (C, D) The expression of osteoblast differentiation-associated genes (RUNX2, OSX, Plzf, Dlx5, and ALP) was assayed by qRT-PCR (C) and western blot (D). NC represents normal control. BL represents low concentration of bilirubin. BM represents medium concentration of bilirubin. BH represents high concentration of bilirubin. Results are displayed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01.

    Article Snippet: Following blocking with TBST solution with 5 % skim milk, primary antibodies against CD63 (1:1000, ab216130, Abcam), TSG101 (1:5000, ab125011, Abcam), Calnexin (1:20000, ab92573, Abcam), RUNX2 (1:1000, 20700-1-AP, Proteintech), OSX (1:1000, 28694-1-AP, Proteintech), Plzf (1:5000, 66672-1-Ig, Proteintech), Dlx5 (1:1000, 10592-1-AP, Proteintech), ALP, (1:200, sc-271431, Santa cruz) and β-actin (1:2000, 60008-1-lg, Proteintech) were appended to incubate membranes overnight at 4 °C.

    Techniques: Staining, Expressing, Quantitative RT-PCR, Western Blot, Control, Concentration Assay

    Auto-exo accelerated osteoblast differentiation by modulating bilirubin. (A) ALP staining. Scale bar = 200 μm. (B) The calcified cells were detected by Alizarin red S staining. Scale bar = 200 μm. (C) The expression of osteoblast differentiation-associated genes (RUNX2, OSX, Plzf, Dlx5, and ALP) was evaluated by qRT-PCR. Results are displayed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01.

    Journal: Biomedical Journal

    Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism

    doi: 10.1016/j.bj.2025.100870

    Figure Lengend Snippet: Auto-exo accelerated osteoblast differentiation by modulating bilirubin. (A) ALP staining. Scale bar = 200 μm. (B) The calcified cells were detected by Alizarin red S staining. Scale bar = 200 μm. (C) The expression of osteoblast differentiation-associated genes (RUNX2, OSX, Plzf, Dlx5, and ALP) was evaluated by qRT-PCR. Results are displayed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01.

    Article Snippet: Following blocking with TBST solution with 5 % skim milk, primary antibodies against CD63 (1:1000, ab216130, Abcam), TSG101 (1:5000, ab125011, Abcam), Calnexin (1:20000, ab92573, Abcam), RUNX2 (1:1000, 20700-1-AP, Proteintech), OSX (1:1000, 28694-1-AP, Proteintech), Plzf (1:5000, 66672-1-Ig, Proteintech), Dlx5 (1:1000, 10592-1-AP, Proteintech), ALP, (1:200, sc-271431, Santa cruz) and β-actin (1:2000, 60008-1-lg, Proteintech) were appended to incubate membranes overnight at 4 °C.

    Techniques: Staining, Expressing, Quantitative RT-PCR

    Auto-exo reduce bone loss and promote osteoblast differentiation in vivo . (A) Schematic diagram of animal experimental design. (B) Quantitative analysis of bone mineral density (BMD) among different groups. (C) HE staining of bone tissues. Scale bar = 100 μm. (D) TRAP staining. Scale bar = 100 μm. (E, F) The expression of osteoblast differentiation-associated genes (RUNX2, OSX, Plzf, Dlx5, and ALP) in OVX mice after Auto-exo treatment were assayed by qRT-PCR (E) and western blot (F). Results are displayed as mean ± SD. ∗ p < 0.05. ∗∗ p < 0.01.

    Journal: Biomedical Journal

    Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism

    doi: 10.1016/j.bj.2025.100870

    Figure Lengend Snippet: Auto-exo reduce bone loss and promote osteoblast differentiation in vivo . (A) Schematic diagram of animal experimental design. (B) Quantitative analysis of bone mineral density (BMD) among different groups. (C) HE staining of bone tissues. Scale bar = 100 μm. (D) TRAP staining. Scale bar = 100 μm. (E, F) The expression of osteoblast differentiation-associated genes (RUNX2, OSX, Plzf, Dlx5, and ALP) in OVX mice after Auto-exo treatment were assayed by qRT-PCR (E) and western blot (F). Results are displayed as mean ± SD. ∗ p < 0.05. ∗∗ p < 0.01.

    Article Snippet: Following blocking with TBST solution with 5 % skim milk, primary antibodies against CD63 (1:1000, ab216130, Abcam), TSG101 (1:5000, ab125011, Abcam), Calnexin (1:20000, ab92573, Abcam), RUNX2 (1:1000, 20700-1-AP, Proteintech), OSX (1:1000, 28694-1-AP, Proteintech), Plzf (1:5000, 66672-1-Ig, Proteintech), Dlx5 (1:1000, 10592-1-AP, Proteintech), ALP, (1:200, sc-271431, Santa cruz) and β-actin (1:2000, 60008-1-lg, Proteintech) were appended to incubate membranes overnight at 4 °C.

    Techniques: In Vivo, Staining, Expressing, Quantitative RT-PCR, Western Blot

    Bilirubin inhibits osteoblast differentiation of MC3T3-E1 cells. (A) ALP staining. (B) The calcified cells were examined by Alizarin red S staining. (C, D) The expression of osteoblast differentiation-associated genes (RUNX2, OSX, Plzf, Dlx5, and ALP) was assayed by qRT-PCR (C) and western blot (D). NC represents normal control. BL represents low concentration of bilirubin. BM represents medium concentration of bilirubin. BH represents high concentration of bilirubin. Results are displayed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01.

    Journal: Biomedical Journal

    Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism

    doi: 10.1016/j.bj.2025.100870

    Figure Lengend Snippet: Bilirubin inhibits osteoblast differentiation of MC3T3-E1 cells. (A) ALP staining. (B) The calcified cells were examined by Alizarin red S staining. (C, D) The expression of osteoblast differentiation-associated genes (RUNX2, OSX, Plzf, Dlx5, and ALP) was assayed by qRT-PCR (C) and western blot (D). NC represents normal control. BL represents low concentration of bilirubin. BM represents medium concentration of bilirubin. BH represents high concentration of bilirubin. Results are displayed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01.

    Article Snippet: Following blocking with TBST solution with 5 % skim milk, primary antibodies against CD63 (1:1000, ab216130, Abcam), TSG101 (1:5000, ab125011, Abcam), Calnexin (1:20000, ab92573, Abcam), RUNX2 (1:1000, 20700-1-AP, Proteintech), OSX (1:1000, 28694-1-AP, Proteintech), Plzf (1:5000, 66672-1-Ig, Proteintech), Dlx5 (1:1000, 10592-1-AP, Proteintech), ALP, (1:200, sc-271431, Santa cruz) and β-actin (1:2000, 60008-1-lg, Proteintech) were appended to incubate membranes overnight at 4 °C.

    Techniques: Staining, Expressing, Quantitative RT-PCR, Western Blot, Control, Concentration Assay

    Auto-exo accelerated osteoblast differentiation by modulating bilirubin. (A) ALP staining. Scale bar = 200 μm. (B) The calcified cells were detected by Alizarin red S staining. Scale bar = 200 μm. (C) The expression of osteoblast differentiation-associated genes (RUNX2, OSX, Plzf, Dlx5, and ALP) was evaluated by qRT-PCR. Results are displayed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01.

    Journal: Biomedical Journal

    Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism

    doi: 10.1016/j.bj.2025.100870

    Figure Lengend Snippet: Auto-exo accelerated osteoblast differentiation by modulating bilirubin. (A) ALP staining. Scale bar = 200 μm. (B) The calcified cells were detected by Alizarin red S staining. Scale bar = 200 μm. (C) The expression of osteoblast differentiation-associated genes (RUNX2, OSX, Plzf, Dlx5, and ALP) was evaluated by qRT-PCR. Results are displayed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01.

    Article Snippet: Following blocking with TBST solution with 5 % skim milk, primary antibodies against CD63 (1:1000, ab216130, Abcam), TSG101 (1:5000, ab125011, Abcam), Calnexin (1:20000, ab92573, Abcam), RUNX2 (1:1000, 20700-1-AP, Proteintech), OSX (1:1000, 28694-1-AP, Proteintech), Plzf (1:5000, 66672-1-Ig, Proteintech), Dlx5 (1:1000, 10592-1-AP, Proteintech), ALP, (1:200, sc-271431, Santa cruz) and β-actin (1:2000, 60008-1-lg, Proteintech) were appended to incubate membranes overnight at 4 °C.

    Techniques: Staining, Expressing, Quantitative RT-PCR