anti plzf (R&D Systems)
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Anti Plzf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+plzf/Human+PLZF+Antibody/pmc13079447-66-28-31
Average 93 stars, based on 89 article reviews
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Blocking Assay:Article Title: Age-Dependent Clonal Expansion of Non-Sperm-Forming Spermatogonial Stem Cells in Mouse Testes. Article Snippet: .. They were then cryo- embedded in OCT compound (4583, Sakura Finetek Japan, Tokyo, Japan), cut into 10- μm sections using a CM1520 cryostat (Leica, Wetzlar, Germany), dried for 30 min, and washed in PBS at 4°C for 20 min. After blocking, the sections were incubated with anti- GFRα1 (1∶1000 dilution), anti- GFP (1:300 dilution; A11122, Invitrogen, Waltham, MA, USA), anti- GFP (1:1000 dilution; ab13970, Abcam, Cambridge, UK), anti- Ki67 (1:1000 dilution; ab15580, Abcam), anti- COPS5 (1:50 dilution; 6895, Cell Signaling Technology), anti- EGR4 (1:50 dilution; ab198197, Abcam), and Article Title: Age‐Dependent Clonal Expansion of Non–Sperm‐Forming Spermatogonial Stem Cells in Mouse Testes Article Snippet: .. They were then cryo‐embedded in OCT compound (4583, Sakura Finetek Japan, Tokyo, Japan), cut into 10‐μm sections using a CM1520 cryostat (Leica, Wetzlar, Germany), dried for 30 min, and washed in PBS at 4°C for 20 min. After blocking, the sections were incubated with anti‐GFRα1 (1∶1000 dilution), anti‐GFP (1:300 dilution; A11122, Invitrogen, Waltham, MA, USA), anti‐GFP (1:1000 dilution; ab13970, Abcam, Cambridge, UK), anti‐Ki67 (1:1000 dilution; ab15580, Abcam), anti‐COPS5 (1:50 dilution; 6895, Cell Signaling Technology), anti‐EGR4 (1:50 dilution; ab198197, Abcam), and Incubation:Article Title: Age-Dependent Clonal Expansion of Non-Sperm-Forming Spermatogonial Stem Cells in Mouse Testes. Article Snippet: .. They were then cryo- embedded in OCT compound (4583, Sakura Finetek Japan, Tokyo, Japan), cut into 10- μm sections using a CM1520 cryostat (Leica, Wetzlar, Germany), dried for 30 min, and washed in PBS at 4°C for 20 min. After blocking, the sections were incubated with anti- GFRα1 (1∶1000 dilution), anti- GFP (1:300 dilution; A11122, Invitrogen, Waltham, MA, USA), anti- GFP (1:1000 dilution; ab13970, Abcam, Cambridge, UK), anti- Ki67 (1:1000 dilution; ab15580, Abcam), anti- COPS5 (1:50 dilution; 6895, Cell Signaling Technology), anti- EGR4 (1:50 dilution; ab198197, Abcam), and Article Title: Undifferentiated spermatogonia modulate their behavior via the expression of basement membrane protein laminin Article Snippet: Sections were blocked for 1 h at room temperature using a solution of 4% donkey serum (Sigma–Aldrich, St. Louis, MO, USA: D9663) in Blocking One (Nacalai Tesque). .. Primary antibodies were incubated overnight at 4°C in 1:1 PBS/Blocking One with anti-GFRα1 (1∶1000 dilution; R&D Systems, Minneapolis, MN, USA: AF560), anti-Lam1 + 2 (1:500 dilution; Abcam: ab7463), Article Title: Age‐Dependent Clonal Expansion of Non–Sperm‐Forming Spermatogonial Stem Cells in Mouse Testes Article Snippet: .. They were then cryo‐embedded in OCT compound (4583, Sakura Finetek Japan, Tokyo, Japan), cut into 10‐μm sections using a CM1520 cryostat (Leica, Wetzlar, Germany), dried for 30 min, and washed in PBS at 4°C for 20 min. After blocking, the sections were incubated with anti‐GFRα1 (1∶1000 dilution), anti‐GFP (1:300 dilution; A11122, Invitrogen, Waltham, MA, USA), anti‐GFP (1:1000 dilution; ab13970, Abcam, Cambridge, UK), anti‐Ki67 (1:1000 dilution; ab15580, Abcam), anti‐COPS5 (1:50 dilution; 6895, Cell Signaling Technology), anti‐EGR4 (1:50 dilution; ab198197, Abcam), and Article Title: DNAJB7 is dispensable for male fertility in mice. Article Snippet: .. Sections were blocked in 10% goat serum and incubated with the following primary antibodies: Article Title: Undifferentiated Spermatogonia Modulate Their Behavior via the Expression of Basement Membrane Protein Laminin†. Article Snippet: Sections were blocked for 1 h at 188 room temperature using a solution of 4% donkey serum (Sigma-Aldrich, St. Louis, MO, USA: D9663) in 189 Blocking One (Nacalai Tesque). .. Primary antibodies were incubated overnight at 4°C in 1:1 PBS/Blocking 190 One with anti-GFRα1 (1∶1000 dilution; R&D Systems, Minneapolis, MN, USA: AF560), anti-Lam1+2 191 (1:500 dilution; Abcam: ab7463), Staining:Article Title: A behind-the-scenes role of BDNF in the survival and differentiation of spermatogonia. Article Snippet: .. TSA Biotin System (Perkin Elmer, Boston, MA, USA) was used to amplify signals for the c-Kit proto-oncogene (KIT) staining, as reported previously.19 The following antibodies were used with indicated dilutions: |
